Animal Imaging and Monitoring

7T MR systems

The Small-Animal MRI/MRS laboratory is a core facility of the Department of Research at Henry Ford Hospital. The core is dedicated to helping other investigators to acquire data in small animals (rats and mice) in an up-to-date 7T MR system from Agilent (formerly Varian). The system includes an Agilent Dual-Drive console with a 4-channel receive ability, RF electronics and coils for imaging rats and mice. Sequences include those for high-resolution T1- and T2- weighted imaging, for diffusion-weighted imaging to construct diffusion tractography images (DTI), and for dynamic studies of blood flow and vascular physiology. This laboratory has all the necessary equipment for high-field in vivo imaging of small animal models of stroke, cerebral trauma, and cerebral tumor. A number of NIH-funded investigators routinely run studies in the laboratory's systems. Attached to the laboratory are personnel well versed in preparing various types of tissues for histological and immunohistochemical analysis. Located in the Basement of Henry Ford Hospital.

Contact information:

Jim Ewing, Ph.D.

jewing1@hfhs.org

(313) 916-2620

Zeiss LSM 510

The confocal microscope core facility has a Zeiss LSM 510 NLO two-photon microscope system that is equipped with a Chameleon-XR Ti:Sapphire laser, which provides femtosecond pulses tuning more than 275nm, from 705nm to 980nm. The system is ideal for imaging fluorescent signals with subcellular resolution from deep tissue in the living small animal.

Contact information:

Zheng Zhang M.D., PhD.

zhazh@neuro.hfh.edu

(313) 916-5456

Caliper LifeSciences Xenogen IVIS Spectrum imaging system

The IVIS Spectrum is the newest imaging system available from Caliper Life Sciences. Like previous IVIS systems, it uses Xenogen's novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. The IVIS Spectrum is the most versatile and advanced in vivo imaging system available on the market today. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas. For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal. Located in E&R 4th floor- Bioresources.

Contact information:

Tom Mikkelsen, M.D.

TMikkel1@hfhs.org

(313) 916-1094

In-Vivo Multispectral FX PRO imaging system

Combines advanced multispectral fluorescence, luminescence, digital X-ray and radioisotopic imaging for in-vivo small animal imaging in a single system. The system's new multispectral tuning of excitation light provides enhanced sensitivity allowing for the identification and separation of multiple fluorophore and the removal of autofluorescence background. The Carestream In-Vivo Multispectral FX PRO automatically generates multispectral fluorophore image "cubes" with spatially co-registered X-ray and white light images for improved localization of biological markers in-vivo.

Contact information:

Ali S. Arbab, M.D., Ph.D.

saali@rad.hfh.edu

(313) 874-4435

Micro-SPECT-CT in vivo small animal imaging system

Combines high-resolution single photon emission computed tomography (SPECT) and X-ray computed tomography (CT) of small animals, such as mouse and rat. The micro-SPECT-CT can be used to acquire images from head to toe. Using FDA-approved clinical radiopharmaceuticals, SPECT-CT images can be obtained to determine both the anatomical and functional images of brain, heart, lungs, kidneys, hepatobiliary, and reticuloendothelial system (RES). High-resolution capability of the CT can be used to image lungs at 10-50 micron thick and allow determining lung volume easily. Using different radioisotopes (such as Tc-99m, Tl-201, In-111, I-125, I-123, or I-131) cell tracking and cancer imaging can be performed with confidence.

Contact information:

Ali S. Arbab, M.D., Ph.D.

saali@rad.hfh.edu

(313) 874-4435