Dr. Shesely had previously developed a gene knockout mouse strain lacking functional endothelial nitric oxide synthase (eNOS or NOS3). He is currently studying the unexpected ability of certain vessels (coronary artery) in eNOS knockout mice to still undergo nitric oxide synthase (NOS) dependent relaxation. In specific the up-regulation of the related neuronal nitric oxide synthase (nNOS or NOS1) as a compensatory mechanism is being investigated. This work is being undertaken at both the transcriptional and functional level making use of various specific NOS knockout mice.
In addition Dr. Shesely is director of a mutant mouse core facility at the Henry Ford Health System for the Division of Hypertension and Vascular Research. This "Core" is currently maintaining 16 distinct colonies of transgenic and gene knockout mice. The Core is responsible for the animal husbandry, maintenance, and genotyping of the mice from the colonies in order to provide the division researchers with genetically modified mice for use in their research.